PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)

Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.

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Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion Flash II DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Color	Colorless
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Invitrogen™ PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Kit
Sample Type	PCR Products
Isolation Technology	Silica Spin Column
Run Time	10 min.

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Thermo Scientific™ Phusion™ Plus DNA Polymerase

Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.

Pricing & Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
Polymerase	Phusion Plus DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>100X
Size (Final Product)	20 kb or less
Reaction Format	Separate components
Reaction Speed	15 to 30 sec./kb
Hot Start	Built-In Hot Start
Overhang	Blunt
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	Not Applicable
Sensitivity	Down to 10 copies
Format	Tube
Passive Reference Dye	None
For Use With (Equipment)	QuantStudio 6 Pro or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier
Sample Type	DNA, RNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Product Line	TaqPath

Thermo Scientific™ PCR Master Mix (2X)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
Concentration	2X
Starting Material	DNA
For Use With (Application)	Standard PCR

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Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Content And Storage	Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Genotyping
PCR Method	qPCR
Multiplex Capability	4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath

Thermo Scientific™ dNTP Set, 100 mM Solutions

Thermo Scientific dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Purity or Quality Grade	>99%
Form	Liquid
Label or Dye	Unlabeled
Concentration	100 mM
For Use With (Application)	Standard PCR,High-fidelity PCR,Real Time PCR (qPCR)

Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
GC-Rich PCR Performance	High
Content And Storage	• Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl₂ solution and DMSO Store at –20°C.
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Separate Components
Product Type	High-Fidelity PCR Kit
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
For Use With (Application)	Standard PCR,High-fidelity PCR

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Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Medium
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR
Polymerase	Phire Hot Start II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Tissue
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

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Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• RevertAid Reverse Transcriptase • RiboLock RNase Inhibitor • 5X Reaction Buffer • dNTP Mix • Oligo(dT)₁₈ Primer • Random Hexamer Primer • Control GAPDH RNA • Forward GAPDH Primer (10 μM) • Reverse GAPDH Primer (10 μM) • Nuclease-free water Store at –20°C.
Reverse Transcriptase	RevertAid
Final Product Type	First-Strand cDNA
For Use With (Application)	RT-PCR
Format	Kit
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

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Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit

Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.

Pricing & Availability

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Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
For Use With (Application)	Hot-start PCR

Applied Biosystems™ TrueMark™ Comprehensive Microbiota Control

The TrueMark Comprehensive Microbiota Control contains a linearized multi-target plasmid pool with microbial target sequences commonly used on Custom OpenArray panels. The control has targets relevant to many microbial profiling applications, including respiratory tract, urinary tract, vaginal microbiota, and antibiotic resistance research.

Pricing & Availability
Specifications

Shipping Condition	Dry ice
Format	Tube
Shelf Life	2 years
Product Line	TrueMark
Detection Method	Primer-probe

Applied Biosystems™ VetMAX™ PRRSV 3.0 Reagents

VetMAX™ PRRSV 3.0 Reagents contain master mix, Xeno RNA control, and a primers/probes mix that enables multiplex, one-step, reverse transcription and real-time PCR (RT-PCR) assays targeting North American (NA) and European (EU) porcine reproductive and respiratory syndrome virus (PRSSV) RNA and Xeno RNA.

Pricing & Availability

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PCR Supplies

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Thermo Scientific™ PCR Master Mix (2X) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ dNTP Set, 100 mM Solutions GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ PCR Master Mix (2X)

Thermo Scientific™ dNTP Set, 100 mM Solutions

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit